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[Preprint]. 2023 Nov 29:2023.11.28.569058. [Version 1] doi: 10.1101/2023.11.28.569058

Figure 4. p62-mediated formation of ubiquitin-rich condensates is required for ATG9A recruitment.

Figure 4.

(A) Confocal imaging of endogenous p62, ubiquitin and endogenously HA-tagged ATG9A in WT, ATG13 KO and ATG13-p62 double KO (dKO) HCT-116 cells (scale bars = 10 μm). Where indicated, cells were treated for 4 hours with 1 uM wortmannin. (B) Quantification of ATG9A-HA and TAX1BP1 colocalization by Pearson’s coefficient Quantitation is from 3 replicates with error bars representing SD. P-values were calculated using a two-tailed student t-test for pair-wise comparison. (C) Quantification of ATG9A-HA and ubiquitin colocalization from 3 biological replicates with error bars representing SD. P-values were calculated using a two-tailed student t-test as in panel B.