Figure 5. Perturb-FISH analysis of the role of ASD risk genes in generating different calcium activity phenotypes in astrocytes.

A) Traces showing the variation in cytoplasmic calcium (y-axis) concentration in example cells (line) across time (x-axis), following ATP stimulation. Variation in calcium concentration shown as difference in fluorescence. Cells are 6 groups using k-means clustering on features extracted from the traces: inactive cells, cells with transients and a high plateau, cells without transients but with a high plateau, cells with large transients, cells without transients and with a low plateau, and finally cells with delayed transients. B) Heatmap showing the enrichment (scale bar) of perturbations in 6 clusters of calcium activity, measured as the ratio between detected and expected frequency, and shown as log fold change in natural log base. C) Volcano plots showing LFC effect sizes (x-axis) versus significance (y-axis) for 6 example perturbations. Yellow indicate significantly affected genes (p<0.05), orange indicates the effect of the perturbation on its target gene. D) Heatmap showing genes with differential expression (x-axis) and their z-scored expression (scale bar) in 6 clusters of calcium activity.