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[Preprint]. 2023 Dec 1:2023.12.01.569075. [Version 1] doi: 10.1101/2023.12.01.569075

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Figure 1. — (A) Sequence alignment of human PAR4 and mouse PAR4. P310 at ECL3 (highlighted in blue) in humans is equal to P322 in mice. (B) gRNAs were targeted against the region of the mouse F2rl3 locus highlighted in red, while the red letters indicate the substitution target site. (C) Sanger sequencing shows the F2rl3 locus around the gRNA target site of a wildtype mouse (top) and a PAR4-P322L homozygous mouse (bottom) in which both alleles contained C>T substitutions. (D) Protein levels of PAR4 were compared across genotypes using an antibody specific for the mouse protein.