Figure 3. Reactivity to non-PAR4 agonists was unaffected in platelets from PAR4-P322L mice.

Platelet rich plasma (PRP) from PAR4^P/P, PAR4^P/L and PAR4^L/L littermates were stimulated with 2.5 – 20 μM ADP (A, B), or 5 nM or 20 nM convulxin (C, D). The α-granule secretion (A, C) and integrin αIIbβ3 activation (B, D) were measured by flow cytometry using P-selectin and activated αIIbβ3 specific antibodies. Data are means ± SD from 5 independent experiments at each concentration. Dots represent individual mice.