Figure 4. Aggregation of platelets from P322L mice had a reduced response to thrombin.

Representative tracing of thrombin-mediated platelet aggregation (A-C). Gel-filtered platelets from PAR4^P/P (A), PAR4^P/L (B) and PAR4^L/L (C) littermates were stimulated with 0.5 – 3 nM thrombin. (D) Maximum aggregation of gel-filtered platelets from PAR4^P/P, PAR4^P/L and PAR4^L/L were compared in response to 0.5 – 3 nM thrombin stimulation. (E) Area under curve of PAR4^P/P, PAR4^P/L and PAR4^L/L platelet aggregation was compared in response to 0.5 – 3 nM thrombin stimulation. (F) The aggregation rate of PAR4^P/P, PAR4^P/L and PAR4^L/L platelet aggregation was compared in response to 0.5 – 3 nM thrombin stimulation. Data are representative of 3 independent experiments. Dots represent individual mice.