Table 1:
Antibodies Used
| Antibody | Company | Catalogue No. | Species | Dilution used (immunoblot/IP) |
|---|---|---|---|---|
| p85α | CST | 4257 | Rabbit | 1:1000 |
| Phospho-AKT/Akt (T308) | CST | 9275 | Rabbit | 1:1000 |
| Phospho-AKT/Akt (S473) | CST | 9271 | Rabbit | 1:1000 |
| AKT/Akt (1,2,3) | CST | 2920 | Mouse | 1:1000 |
| p110α | CST | 4249 | Rabbit | 1:1000/1:50 |
| p110δ | CST | 34050 | Rabbit | 1:1000 |
| Irs 1 | Millipore | 06–248 | Rabbit | 1:500 |
| Irs 1 | CST | 2382 | Rabbit | 1:1000/1:50 |
| Irs2 clone 9.52 | Millipore | MABS15 | Mouse | 1:1000/1:50 |
| β-actin | CST | 4967 | Rabbit | 1:5000 |
| Anti-rabbit IgG; HRP-linked | CST | 7074 | Goat | 1:5000 |
| Anti-mouse IgG; HRP-linked | CST | 7076 | Horse | 1:5000 |