FIG. 3.

Primer extension analysis of bldB transcript. An oligonucleotide complementary to sequence immediately downstream of the putative translation start site (shown in Fig. 2) was used for extension reactions. Lane 1 is a tRNA control. RNA was isolated from J701 containing pMP1.6 after 2 days of growth (lane 2); J701 after 2 days of growth (lane 3); J704 after 2 days of growth (lane 4); J704 containing pMP573 after growth for 6 days (lane 5), 2 days (lane 6), or 1 day (lane 7); and A3(2) after growth for 3 days (lane 8), 2 days (lane 9), or 1 day (lane 10).