FIG. 4.

Expression from the bldB-xylE fusion in wild-type and bldB mutant strains. The histogram shows results of catechol dioxygenase assays (specific activity) from cells grown for 1, 2, or 3 days on either glucose or mannitol as the carbon source. Expression of the bldB-xylE fusion was examined in an S. coelicolor wild-type strain A3(2) and J701, which contains the bldB15 allele. Specific activity is expressed as the rate of change in optical density at 375 nm per minute per milligram of protein. Duplicate assays gave nearly identical results and so error bars are not visible.