Table 2.

Conditions for the multistep EDC/NHS reactions, including the pH, buffers, and temperature during the conjugation of nanogels with targeting ligand.

Reaction Scheme	pH at Activation	Buffer Used	pH at Conjugation	Buffer Used	Temperature
M1	not controlled	-(pure water)	7.4	PBS	4 °C
M2			8.2	0.1 M TES buffer
M3	pH 5.5	0.1 M MES buffer	7.4	PBS
M4			8.2	0.1 M TES buffer
M5	pH 6.3	0.1 M MES buffer	7.4	PBS
M6			8.2	0.1 M TES buffer