Table 6.
Protein | Primary Antibody | Secondary Antibody |
---|---|---|
GAPDH | Mouse monoclonal anti-GAPDH antibody (overnight at 4 °C, 1:10,000 dilution; sc-59540, Santa Cruz Biotechnology Inc., Dallas, TX, USA) | Alkaline phosphatase-conjugated goat anti-mouse polyclonal antibody (4 h at 4 °C, 1:15,000 dilution; A3688, Sigma Aldrich, St. Louis, MO, USA) |
GRPR | Rabbit polyclonal anti-GRPR antibody (overnight at 4 °C, 1:1000 dilution; PA5-33833, Thermo Fisher Scientific Inc., Waltham, MA, USA) |
Alkaline phosphatase-conjugated goat anti-rabbit polyclonal antibody (4 h at 4 °C, 1:15,000 dilution; A3687, Sigma Aldrich, St. Louis, MO, USA) |