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. 2023 Nov 29;15(23):5646. doi: 10.3390/cancers15235646

Table 6.

Antibodies used for Western blotting.

Protein Primary Antibody Secondary Antibody
GAPDH Mouse monoclonal anti-GAPDH antibody (overnight at 4 °C, 1:10,000 dilution; sc-59540, Santa Cruz Biotechnology Inc., Dallas, TX, USA) Alkaline phosphatase-conjugated goat anti-mouse polyclonal antibody (4 h at 4 °C, 1:15,000 dilution; A3688, Sigma Aldrich, St. Louis, MO, USA)
GRPR Rabbit polyclonal anti-GRPR antibody
(overnight at 4 °C, 1:1000 dilution;
PA5-33833, Thermo Fisher Scientific Inc., Waltham, MA, USA)
Alkaline phosphatase-conjugated goat anti-rabbit polyclonal antibody (4 h at 4 °C, 1:15,000 dilution; A3687, Sigma Aldrich, St. Louis, MO, USA)