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. 2023 Nov 25;24(23):16762. doi: 10.3390/ijms242316762

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Map-based cloning of the YGL19 gene. (A) Preliminary mapping of the YGL19 gene. The YGL19 locus was initially mapped to a 3.3 Mb region between two markers, chr03MM2192 and chr03MM2665, on chromosome 3. (B) Fine mapping of the YGL19 gene. The locus was further mapped within an 84 kb region between the 3M08143 and 3M08189 markers. Numbers below the line indicate the number of F₂ recombinants at the marker regions. (C) The C to T substitution of the YGL19 gene in ygl19 mutant. Yellow and blue squares represent the CDS and upstream/downstream region, respectively. The black line represents the intron. The red arrow indicates the C to T substitution position in ygl19 mutant. The codon changed from CCC to TCC, and the amino acid coding changed from Proline (Pro) to Serine (Ser).