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. 2023 Nov 25;24(23):16762. doi: 10.3390/ijms242316762

Figure 12.

Figure 12

Phenotype of ROS accumulation in wild-type and ygl19 mutant plant leaves at the tillering stage. (A–C) Histochemical detection of superoxide anions via NBT staining, hydrogen peroxide via DAB staining, and dead cells via trypan blue staining. (D) MDA content. (E) Relative expression of genes related to ROS scavenging. (F) Relative expression of ROS-responsive genes. WT, wild type. In (D–F), error bars represent the SDs of three biological replicates, and asterisks indicate statistically significant differences in the ygl19 mutant compared with the wild type (** p < 0.005, *** p < 0.0005, Student’s t-test).