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. 2023 Nov 24;24(23):16689. doi: 10.3390/ijms242316689

Figure 3.

Figure 3

Probable inhibition of ER transactivities in GCs of PCOS. (A) GCs of PCOS and control women were fixed and permeabilized, and the localization of ERα, ERβ2 and ERβ5 proteins were monitored via immunofluorescence using anti-ER (α, β2, or β5) antibodies (red). Nuclei were stained with DAPI (blue). Representative images at 20× magnification are presented. The experiment was also performed on HGrC1 cell line as negative control. (B) RNA extraction was performed from GCs of PCOS and control women. Relative levels of coregulators, NCOA1 and NCOR1 mRNA, were determined via RT-qPCR analysis for each group. Transcript levels were normalized to GAPDH transcript abundance. Values are represented as means ± SEM (n = 5), measured in triplicate. Differences between groups were considered significant for p ≤ 0.05 *, via Mann–Whitney test. A.U., Arbitrary Units.