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. 2023 Nov 23;24(23):16648. doi: 10.3390/ijms242316648

Figure 1.

Figure 1

Effect of Ptx3 genetic deficiency on the osteogenic properties of OBs. (A) Analysis of Ptx3 expression in primary OBs from calvariae of WT mice in basal condition and at different timepoints during osteogenic induction. Ptx3 mRNA levels were determined by qPCR. Results were normalized based on Gapdh expression and reported as arbitrary units (AU); * p < 0.05. (B) PTX3 protein was quantified in the supernatant of WT OB cultures by ELISA (3 independent experiments performed in duplicate, n = 6, mean ± SEM). One-way ANOVA with multiple comparison test. (C) Assessment of in vitro collagen production by WT and Ptx3−/− OBs stimulated with ascorbic acid for 8 h. Cultures were stained with Sirius red; representative images are shown. Afterwards, staining was extracted, and absorbance measured at 540 nm. Mann–Whitney test. (D) Time-course analysis of mineralization in WT and Ptx3−/− primary OB cultures, as assessed by Alizarin red staining (ARS) and quantification at 405 nm and expression of osteogenic marker genes. One-way ANOVA with multiple comparison test; * p < 0.05. All the data presented in this figure are derived from 3 independent experiments performed in duplicate, n = 6, and are represented as mean ± SEM.