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. 2023 Nov 23;24(23):16648. doi: 10.3390/ijms242316648

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) FACS analysis of the indicated surface markers on WT and Ptx3^−/− KO OBs in basal condition (not treated with oim). The percentage of CD45^- cells expressing each marker as well as the mean fluorescence intensity for each marker in both genotypes is indicated as mean ± SEM. Mann–Whitney test; * p < 0.05. (B) Gene expression analysis of the hyaluronic acid synthases Has1, Has2, and Has3 and Tnfaip6 gene in WT and Ptx3^−/− OBs at different timepoints during osteogenic induction; mRNA levels were determined by qPCR and normalized on Gapdh expression, and they are indicated as arbitrary units (AU) (3 independent experiments performed in duplicate, n = 6, mean ± SEM). One-way ANOVA.