Figure 3.
C5aR2 KO amplifies expression and phosphorylation of cGAS-STING pathway proteins. PMA-differentiated wild-type or C5aR2 KO THP-1 cells were stimulated with 5 μg/mL STING agonist cAIM(PS)2 Difluor (Rp/Sp) for 6 h. (A,B) Lysates were assessed for expression and phosphorylation of cGAS-STING pathway proteins STING and IRF3 by Peggy Sue. (C–G) Area under the curve for each protein was normalised to β-actin. Mean ± SD. N = 3 wild-type replicates and C5aR2 KO clones represented by shapes; Circle: D3. Square: F3. Triangle: F7. Grey; NTC, Red; C5aR2 edited, light; vehicle, dark; agonist.