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SDS-PAGE (12% polyacrylamide) of crude extracts of S. lividans(pIJ699) (lane 1) and S. lividans(pMETF150) (lane 2). Cultures were grown in NMMP medium (16) supplemented with thiostrepton (5 μg/ml). Lane M, SDS-PAGE molecular weight standards (low range; Bio-Rad). Sizes are indicated on the left. The overexpressed protein is indicated with an arrow.
