TABLE 1.
Plasmids used in this study
| Plasmid | Relevant features | Source or reference |
|---|---|---|
| pIJ2921 | 2.7-kb E. coli plasmid; lacZ complementation; bla | 17 |
| pBluescript | 2.9-kb E. coli phagemid; lacZ complementation; bla (sequencing vector) | Stratagene |
| pIJ699 | 9.6-kb bifunctional E. coli-Streptomyces positive selection vector; tsr aph vph | 20 |
| pGM7 | 4.95-kb Streptomyces temperature-sensitive replication plasmid; tsr (gene disruption vector) | 24 |
| pULVK99 | 7.8-kb bifunctional E. coli-Streptomyces positive selection vector; tsr kan | 4 |
| pMETF1 | pIJ2921 carrying a 10-kb PstI DNA fragment of S. lividans containing metF (original clone) | This work |
| pMETF50 | pIJ2921 carrying a 1.4-kb KpnI DNA fragment containing metF | This work |
| pMETF100 | pIJ2921 carrying a 255-bp SalI DNA fragment internal to metF | This work |
| pMETF150 | pIJ699 carrying a 1.4-kb BglII DNA fragment derived from pMETF50 and containing metF | This work |
| pMETF200 | pGM7 carrying a 0.3-kb BglII DNA fragment derived from pMETF100 and containing the 255-bp SalI DNA fragment internal to metF | This work |
| pVKK-metF | pULVK99 carrying a 1.4-kb BglII DNA fragment derived from pMETF50 and containing metF | This work |