FIG. 2.

Physical map of the DNA fragment carrying the T308 mutant locus and localization of the BD413 competence factor, comC (large arrow), by subcloning and complementation analysis. Plasmids are named on the left. The direction of transcription from the lac promoter is indicated by the small arrows. Transformability was tested by demanding acquisition of the pobA wild-type allele from BD413 DNA during growth of ADP239 (pobA mutant) transconjugants carrying pRT308, pRK1, pRK2, pRK3, pRK9, or pCL20, with p-hydroxybenzoate as the sole carbon source. −, transformability not restored; +, transformability restored.