Figure 2.

Galgravin attenuates the LPS-induced elevation of iNOS and COX-2 in RAW 264.7 cells. (A) RAW 264.7 cells (1 × 10⁶ cells/well in MP-6) were pretreated with a vehicle (0.1% DMSO) or different concentrations of galgravin for 1 h and then incubated with LPS (50 ng/mL) for 24 h. Upon lysis, protein lysates (30 μg) were resolved using 10% SDS-PAGE and subjected to Western blot analysis to determine the expression levels of iNOS and COX-2. (B) The quantification data of iNOS from three independent experiments are shown as the mean ± SEM. (C) The quantification data of COX-2 from three independent experiments are shown as the mean ± SEM. The asterisk indicates significant differences from the LPS-treated vehicle control (*, p < 0.05; **, p < 0.01). iNOS, inducible nitric oxide synthase. COX-2, cyclooxygenase 2.