Table 6.
Insect | Protease(s) | Enzyme/Substrate Ratio |
Temperature (°C) |
Time | pH | Speed (rpm) |
Reference | |
---|---|---|---|---|---|---|---|---|
1 | Tropical house cricket (Gryllodes sigillatus) | Alcalase | 0.5–3.0% | 50 °C | 0.5–1.5 h | 8.0 | - | [88] |
2 | Mealworm larva (Tenebrio molitor (L.)) | Alcalase | 1:100 | 50 °C | 4.0 h (250 min) |
8.5. | [89] | |
3 | Silkworm pupae (Bombyx mori) | Alcalase® Prolyve® Flavourzyme® Brewers Clarex® |
1% (w/v) | 50 °C | 4.0 h | 8.0 | [90] | |
4 | Tropical house cricket (Gryllodes sigillatus) | Alcalase | 3.0% | 55 °C | 80 min | 8.0 | [91] | |
5 | Black crickets (G. assimilis) | FlavourzymeTM, Alcalase Neutrase |
100 mg/mL | 50 °C | 4.0 h | 7.0 | 100 | [92] |
6 | Mealworm (Tenebrio molitor) | Alcalase® Pepsin |
0.03% (w/w) 0.25% |
60 °C 40 °C |
2.0 h 4.0 h |
8.5 2.0 |
[93] | |
7 | Lesser mealworm (Alphitobius diaperinus L.) | Enzymatic extract of mature artichoke flower |
1% (w/v) | 50 °C | 16.0 h | 6.2 | [94] | |
8 | Mealworm larvae (Tenebrio molitor), Criket (Gryllus bimaculatus), Silkworm pupae (Bombyx mori) |
Flavourzyme Alcalase Neutrase Protamex |
30 and 60 U/g protein 12 and 72 mU/g protein 4 and 24 mU/g protein 7.5 and 45 mU/g protein |
55 °C | 8.0 h | [84] | ||
9 | Lesser mealworm (Alphitobius diaperinus) | Alcalase Corolase |
0.5–3.0% 0.5–3.0% |
50 °C | 0.0–24.0 h | 8 | [83] | |
10 | Lesser mealworm (Alphitobius diaperinus) Black soldier fly larvae (Hermetia illucens) |
Papain Pancreatin Dispase I Pepsin Protease From Bacillus Licheniformis Bromelain Trypsin |
1:10 | 60 °C 37 °C 37 °C 37 °C 60 °C 50 °C 37 °C |
18.0 h | 6.5 7.8 7.3 3.0 7.5 7.0 7.8 |
[80] | |
11 | White-spotted flower chafer (Protaetia brevitarsis) | Subcritical water | --- | 100–300 °C | --- | --- | [87] |