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. 2023 Nov 24;12(23):4243. doi: 10.3390/foods12234243

Table 6.

Different conditions and enzymes used for hydrolysis of various insects’ proteins.

Insect Protease(s) Enzyme/Substrate
Ratio
Temperature
(°C)
Time pH Speed
(rpm)
Reference
1 Tropical house cricket (Gryllodes sigillatus) Alcalase 0.5–3.0% 50 °C 0.5–1.5 h 8.0 - [88]
2 Mealworm larva (Tenebrio molitor (L.)) Alcalase 1:100 50 °C 4.0 h
(250 min)
8.5. [89]
3 Silkworm pupae (Bombyx mori) Alcalase®
Prolyve®
Flavourzyme®
Brewers Clarex®
1% (w/v) 50 °C 4.0 h 8.0 [90]
4 Tropical house cricket (Gryllodes sigillatus) Alcalase 3.0% 55 °C 80 min 8.0 [91]
5 Black crickets (G. assimilis) FlavourzymeTM,
Alcalase
Neutrase
100 mg/mL 50 °C 4.0 h 7.0 100 [92]
6 Mealworm (Tenebrio molitor) Alcalase®
Pepsin
0.03% (w/w)
0.25%
60 °C
40 °C
2.0 h
4.0 h
8.5
2.0
[93]
7 Lesser mealworm (Alphitobius diaperinus L.) Enzymatic extract of
mature artichoke flower
1% (w/v) 50 °C 16.0 h 6.2 [94]
8 Mealworm larvae (Tenebrio molitor),
Criket (Gryllus bimaculatus), Silkworm pupae
(Bombyx mori)
Flavourzyme
Alcalase
Neutrase
Protamex
30 and 60 U/g protein
12 and 72 mU/g protein
4 and 24 mU/g protein
7.5 and 45 mU/g protein
55 °C 8.0 h [84]
9 Lesser mealworm (Alphitobius diaperinus) Alcalase
Corolase
0.5–3.0%
0.5–3.0%
50 °C 0.0–24.0 h 8 [83]
10 Lesser mealworm (Alphitobius diaperinus)
Black soldier fly larvae (Hermetia illucens)
Papain
Pancreatin
Dispase I
Pepsin
Protease From Bacillus Licheniformis
Bromelain
Trypsin
1:10 60 °C
37 °C
37 °C
37 °C
60 °C
50 °C
37 °C
18.0 h 6.5
7.8
7.3
3.0
7.5
7.0
7.8
[80]
11 White-spotted flower chafer (Protaetia brevitarsis) Subcritical water --- 100–300 °C --- --- [87]