Autophagy inhibition in CyDs-treated AML cells. (A,B) Effects of chloroquine (20 μM), LY294002 (50 μM), and bafilomycin A1 (1 nM) on the antitumor activity of FA-HP-β-CyD (A) and HP-β-CyD (B) in HL-60 cells. (C,D) Effects of chloroquine (20 μM), LY294002 (50 μM), and bafilomycin A1 (1 nM) on the antitumor activity of FA-HP-β-CyD (A) and HP-β-CyD (B) in THP1 cells. Cells were treated with both 1 mM of FA-HP-β-CyD and 10 mM of HP-β-CyD at 37 °C for 2 h. Then, cells were incubated with the compounds for 24 h. Cell proliferation was assessed using Cell Counting Kit-8. Results are presented as the mean ± SEM (n = 3). * p < 0.05, compared with FA-HP-β-CyD without inhibitor.