Figure 6.

Combined effects of FA-HP-β-CyD and Ara-C or Venetoclax. (A–C) FA-HP-β-CyD was combined with Ara-C. (D–F) FA-HP-β-CyD was combined with Venetoclax. Cells were incubated for 72 h with five concentrations (0.25-, 0.5-, 0.75-, 1.0-, 1.5-fold the IC₅₀) of each agent, or both in combination, using the constant ratio design, followed by modified MTT assay (Section 4.4). The IC₅₀ values of Ara-C for HL-60, THP1 and SKM1 cells were 0.495 μM, 7.19 μM and 0.6 µM, respectively, and those of Venetoclax were 50.3 nM, 441.8 nM and 1.1 µM, respectively. The combination index (CI) was calculated using Calcusyn and plotted as a function of the fraction affected (Fa). For example, 50% growth inhibition would result in a Fa of 0.5. The synergistic (CI < 1), additive (CI = 1), or antagonistic (CI > 1) effects of combining multiple equal concentrations of drugs was assessed. Data are presented as the mean ± SEM of three independent experiments.