Figure 7.

Effect of FA-HP-β-CyD on survival in leukemia mouse models and its effect in combination with Ara-C or Venetoclax. (A,B) Survival of mice transplanted with HL-60 cells (A) and THP12 cells (B). In these groups, the effect of Ara-C combination was also evaluated. HL-60 cells (5 × 10⁶) or THP1 cells (5 × 10⁶) were injected into BRJ mice. Three days later, mice received 200 μL of vehicle, 150 mM (2086.5 mg/kg) of HP-β-CyD, or 15 mM (249 mg/kg) of FA-HP-β-CyD via intraperitoneal injection (twice a day). Ara-C (100 mg/kg) was also injected intraperitoneally once a day. Administration continued for 20 days, and survival was checked daily. Black lines, yellow lines, blue lines, red lines, and green lines indicate survival of mice treated with vehicle, Ara-C, HP-β-CyD, FA-HP-β-CyD, and Ara-C plus FA-HP-β-CyD, respectively. (C) Survival curves of mice transplanted with HL-60 cells. In this group, the effect of Venetoclax combination was also evaluated. HL-60 cells (5×10⁶) were injected into BRJ mice. Three days after the injection, mice received 200 μL of vehicle, 150 mM (2086.5 mg/kg) of HP-β-CyD, or 15 mM (249 mg/kg) of FA-HP-β-CyD via intraperitoneal injection (twice a day). Venetoclax (25 mg/kg) was given daily by oral gavage. Administration was continued for 20 days, and survival was checked daily. Black lines, yellow lines, blue lines, red lines, and green lines indicate survival of mice treated with vehicle, Venetoclax, HP-β-CyD, FA-HP-β-CyD, and Venetoclax plus FA-HP-β-CyD, respectively. Survival data were analyzed using a log-rank nonparametric test and are shown as Kaplan–Meier survival curves (n = 6). * p < 0.05 and ** p <0.01 compared with vehicle group. Median survival time (day) for each group is shown.