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. 2023 Nov 29;12(23):4316. doi: 10.3390/foods12234316

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of the extracts on intracellular ROS in LPS-induced RAW 264.7 macrophages. (a) Assessment of ROS levels by confocal laser scanning microscopy with DCFH-DA fluorescent dye. ROS levels were evaluated by confocal laser scanning microscopy with DCFH-DA fluorescent dye. Green: DCF fluorescent representation of intracellular ROS. Scale: 100 μm. (b) Quantitative analysis of DCF fluorescence intensity percentage in bar graphs. Different letters (a–e) mean significantly different at p < 0.05. Note: control: without-H₂O₂ treatment group; blank: group treated with only 200 μM H₂O₂.

Effects of the extracts on intracellular ROS in LPS-induced RAW 264.7 macrophages. (a) Assessment of ROS levels by confocal laser scanning microscopy with DCFH-DA fluorescent dye. ROS levels were evaluated by confocal laser scanning microscopy with DCFH-DA fluorescent dye. Green: DCF fluorescent representation of intracellular ROS. Scale: 100 μm. (b) Quantitative analysis of DCF fluorescence intensity percentage in bar graphs. Different letters (a–e) mean significantly different at p < 0.05. Note: control: without-H₂O₂ treatment group; blank: group treated with only 200 μM H₂O₂.