Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 4;12(23):2765. doi: 10.3390/cells12232765

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Analysis of the PDGFb transgene-expressing cells in the spleen. (A) To detect the expression pattern of the PDGFb construct in spleen cells, PDGFb transgene reporter (GFP) was detected using FACS analysis on spleen samples. GFP-positive cells (gate #A2) were mainly CD41⁺/CD150⁺ (gate #A4, #A5) showing (B) big cell volume and high granularity (gate #A6) indicating megakaryocytic lineage properties. There was the rest of unidentified GFP⁺/CD41^- cells, which were detected by FACS (gate #A3) as well as by (C) immunofluorescent staining on cryosections. Blue, DAPI; green, GFP; red, CD41. (D) Cre^⊝ CTL spleen cells stained for GFP. (E) Fluorescence minus one (FMO) control for CD41-PE/Cy7. (F) Fluorescence minus one (FMO) control for CD150-PE.