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. 2023 Dec 4;12(23):2765. doi: 10.3390/cells12232765

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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FACS analysis of hematopoietic stem cells (HSCs) and progenitors in the BM. (A) BM-gating strategy: KSL cells (A4: LT-HSCs, ST-HSCs, MPPs) and KL cells (A3: CMPs, GMPs, MEPs) were gated out of lin^- cells (A2); 1 × 10⁶ events were recorded using FACS ARIA. (B) HSCs, (C) granulocyte-monocyte (GMPs), (D) common myeloid (CMPs) and (E) megakaryocyte–erythrocyte (MEPs) progenitors in the BM of Ctnnb1^BM-CTL (n = 9), Ctnnb1^BM-GOFw/fl (n = 4) and Ctnnb1^BM-GOFfl/fl (n = 7) mice after tamoxifen treatment. Significance is indicated as follows: ***, p < 0.001.