Table 1.
Antibodies.
| Antibody | Host | Company | Catalog # | Dilution | Method |
|---|---|---|---|---|---|
| ABs for Immunofluorescence and Immunohistochemistry | |||||
| GFP | Chicken | Abcam (Cambridge, UK) | ab13970 | 1:500 | PFA |
| CD41 | Rat | BD Biosciences (Heidelberg, Germany) | 553847 | 1:200 | PFA |
| Ki67 (SP6) | Rabbit | ThermoFisher Scientific (Darmstadt, Germany) | RM-9106S0 | 1:200 | PFA |
| Donkey α Rat 550 | Donkey | ThermoFisher Scientific (Darmstadt, Germany) | SA5-10027 | 1:200 | PFA |
| Goat α Chicken 488 | Goat | Abcam | Ab150169 | 1:200 | PFA |
| ABs for flow cytometry (FACS) | |||||
| CD3-PE/Cy7 | BioLegend (Amsterdam, The Netherlands) |
100220 | 1:70 | ||
| CD4- PerCP | BD Biosciences (Heidelberg, Germany) | 553052 | 1:50 | ||
| CD11b-v450 | BD Biosciences (Heidelberg, Germany) | 560456 | 1:50 | ||
| CD16/32-PE/Cy7 | BioLegend | 101318 | 1:75 | ||
| CD19-PE | BD Biosciences (Heidelberg, Germany) | 557399 | 1:50 | ||
| CD34-PE | BD Biosciences (Heidelberg, Germany) | 551387 | 1:75 | ||
| CD41- PE/Cy7 | BioLegend (Amsterdam The Netherlands) |
133916 | 1:50 | ||
| CD45.1-PerCP/Cy5.5 | BioLegend (Amsterdam The Netherlands) |
110727 | 1:70 | ||
| CD45.2-BV510 | BioLegend (Amsterdam The Netherlands) |
109837 | 1:70 | ||
| CD150-PE | BioLegend (Amsterdam The Netherlands) |
115904 | 1:50 | ||
| c-Kit-APC | BD Biosciences (Heidelberg, Germany) | 553356 | 1:75 | ||
| F4/80-APC | eBioscience (Darmstadt, Germany) | 17-4801-82 | 1:50 | ||
| GFP-Alexa 488 | Life Technologies (Darmstadt, Germany) | A-21311 | 1:400 | PFA | |
| Lineage Panel: (TER-119, CD11b, Ly-6G/Ly-6C, CD3e, CD45R/B220)-Biotin | BioLegend (Amsterdam The Netherlands) |
133307 | 1:300 | ||
| Ly6G-FITC | BD Biosciences (Heidelberg, Germany) | 551460 | 1:50 | ||
| Sca-1-PB | BioLegend (Amsterdam The Netherlands) |
122520 | 1:75 | ||
| Streptavidin-PerCP/Cy5.5 | BioLegend (Amsterdam The Netherlands) |
405214 | 1:300 | ||