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Pyruvylation of the lipid-linked intermediate in gumL and wild-type strains. Incubations were performed in the presence of UDP-[14C]glucuronic acid, GDP-mannose, UDP-glucose, and PEP. Radioactive material released from 1203 extracts by mild-acid hydrolysis was submitted to paper electrophoresis. X. campestris FC2 (A) and XcL (B) were employed. AMP and UMP were added as internal standards.
