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Western blot reactivity of monoclonal antibody H1337 to fragments 1 to 5. The E. coli lysates containing pGEX-encoded glutathione S-transferase or a product from pTOPE bearing an irrelevant insert were used for negative controls in lanes Ec1 and Ec2, respectively. Fragments 1 to 5 are described in the legend to Fig. 2. Reactivity of H1337 to P66 of B. burgdorferi B31 is shown in the rightmost lane (Bb). MWS, molecular weight standards (in thousands).
