Sequence comparison of predicted surface-exposed region and flanking transmembrane regions of P66 from Lyme disease Borrelia spp., relapsing fever Borrelia spp., B. coriaceae, and B. anserina. The surface-exposed segment is delimited from the transmembrane regions by spaces. Gaps are indicated by hyphens. Consensus (Cons) amino acids at each position were those that occurred in at least six of the eight sequences. Amino acids are numbered according to the processed P66 sequence of B. burgdorferi B31. Bb, B. burgdorferi B31; Baf, B. afzelii ACAI; Bg, B. garinii Ip90; Bh, B. hermsii; Bp, B. parkeri; Bt, B. turicatae; Bc, B. coriaceae; Ban, B. anserina.