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. 2023 Nov 30;24(23):16974. doi: 10.3390/ijms242316974

Figure 4.

Figure 4

Histological analysis of the leaf blades, stems, and grains between WT and spo1 mutant plants. (a) Cross sections of leaf blades of WT and spo1 mutant. (b) Cross section of midrib, large vein, and small vein at the middle of the second leaf from the top of WT and spo1 mutant at tillering stage. (c–g) Statistics analysis of a number of large veins (c) and small veins (d) per leaf, width of veins (e), number of BCs (f), area of BCs (g). (h) Longitudinal sections of stems between WT and spo1 mutant. (i) The length of stem cells. (j) The area of stem cells. (k) Grain morphology and scanning electron microscopy of the outer surfaces of WT and spo1 glumes. (l,m) Cell width (l) and length (m) of outer glume of the grain. At least 30 samples of WT and spo1 mutant were measured for each. Data are means ± SD, asterisks indicate significant differences according to Student’s t-test (* p < 0.05; ** p < 0.01, ns means no significance). Abbreviations: Ab, abaxial surface; Ad, adaxial surface; BC, bulliform cell; EC, epidermal cell; LV, large vein; SV, small vein. Scale bars: (a) 500 μm; (b) 100 μm; (h) 100 μm; (k) 0.2 cm and 50 μm.