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Experimental design. Forty-eight male Fischer 344 rats were randomly divided into two groups based on their diet: 24 were fed an STD and the other half were fed a CAF for 6 weeks. Then, the animals were abruptly transferred from L12 to L6 or L18 photoperiods, and treatment with VH or GSPE diluted in VH at a dose of 25 mg/kg b.w. was administered. After a week, the rats were euthanized, and serum and liver were collected for further analysis.
