FIG. 7.
Effects of HrcA on GroEL and DnaK syntheses during heat shock. Cells of C. crescentus NA1000 (hrcA+) or LS2293 (hrcA) were subjected to heat shock at 40°C, and aliquots of cells were pulse-labelled with [35S]methionine for 2 min at the indicated times (in minutes). Cells were then harvested by centrifugation, and protein extracts were immunoprecipitated with anti-GroEL or anti-DnaK antibodies. The relative rates of GroEL and DnaK syntheses were determined by densitometry scanning of the autoradiograms. Symbols: ○, hrcA+; ▪, hrcA.