Table 2.
Software for pegRNA design.
| Application | Off-Target Effects | PAMless Cas9 Variants | Web Address | Reference |
|---|---|---|---|---|
| Prime Editing Design Tool | Yes | Yes |
https://primeedit.nygenome.org/ (Accessed on 19 November 2023) |
[20] |
| pegFinder | No | Yes |
http://pegfinder.sidichenlab.org/ (Accessed on 19 November 2023) |
[21] |
| PrimeDesign | Yes | No |
http://primedesign.pinellolab.org/ (Accessed on 19 November 2023) |
[22] |
| CRISPRseek | Yes | No |
https://bioconductor.org/packages/release/bioc/html/CRISPRseek.html (Accessed on 19 November 2023) |
[23] |
| multicrispr | Yes | No |
https://www.bioconductor.org/packages/release/bioc/html/multicrispr.html (Accessed on 19 November 2023) |
[24] |
| Prime Induced Nucleotide Engineering Creator of New Edits (PINECONE) |
Yes | No |
https://github.com/xiaowanglab/PINE-CONE (Accessed on 19 November 2023) |
[25] |
| PE-Designer | Yes | Yes |
http://www.rgenome.net/pe-designer/ (Accessed on 19 November 2023) |
[26] |
| PnB designer | No | No |
https://fgcz-shiny.uzh.ch/PnBDesigner/ (Accessed on 19 November 2023) |
[27] |
| pegIT | Yes | Yes |
https://pegit.giehmlab.dk/ (Accessed on 19 November 2023) |
[28] |
| Easy-Prime | Yes | No | http://easy-prime.cc/ (Accessed on 19 November 2023) | [29] |
| PlantPegDesigner | No | Yes |
https://github.com/JinShuai001/PlantPegDesigner (Accessed on 19 November 2023) |
[30] |