FIG. 3.
Immunoblot and fluorograph of Pho81, the htrVIII++/htrVIII+-1 strain, and the ΔhtrVIII-24 strain. (A) Western blotting analysis with HC23 antibody. Lanes: 1, Pho81; 2, the ΔhtrVIII-24 strain; 3, the htrVIII++/htrVIII+-1 strain. (B) Electrophoretic analysis of methyl-3H-labeled transducers. Lanes: 1, Pho81; 2, the ΔhtrVIII-24 strain; 3, the htrVIII++/htrVIII+-1 strain. The molecular mass is in kilodaltons. Arrows indicate the position of HtrVIII.