Figure 6:

Vascular damage created by doxorubicin treatment. a) Sample vessel images of endothelial (red) treated with or without doxorubicin using TACE and IV methods. Sample images are from HepG2 HCCoCs with native stiffness and normal state. Quantified vascular porosity change with and without the influence of doxorubicin delivered by IV and TACE methods on b) HepG2 and c) HepG2-CYP3A4 HCC cells. Nonselective vascular porosity increases 24 h after 10 μM doxorubicin was delivered using IV or TACE methods. *p < 0.05, **p < 0.01. n = 4. All data represent means ± SD. Scale is 300 μm. IV: Intravenous. TACE: transcatheter arterial chemoembolization. Inf.: Inflammation, Cirr: Cirrhosis, Dox: Doxorubicin