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. 2023 Nov 29;24(23):16953. doi: 10.3390/ijms242316953

Figure 4.

Figure 4

The relationship between PWWP2A mutation and melanin deposition in zebrafish. (A) Detection of the PWWP2A transcript by whole-mount in situ hybridization. hpf, hours post-fertilization. (B) Sequencing results of the PWWP2A gene of wild-type and mutant zebrafish. (C) Relative mRNA levels of PWWP2A in WT and heterozygous mutants were assayed by RT-PCR at 24, 48, and 72 hpf. The results are expressed as the mean ± SEM of three independent experiments (* p < 0.05, ** p < 0.01; t test). (D–F) Phenotypic comparison of mutant zebrafish and wild-type zebrafish at 36, 48, and 72 hpf. Dozens of zebrafish in the same batch were divided into three groups according to the melanin content of the lateral line, and the number in the figure represents the number of zebrafish in each group.