Figure 13.

Status of β-catenin and GSK3β and their complexes with PS1 in A360T fibroblasts. The content of active non-phosphorylated β-catenin was decreased in A360T cells compared to healthy controls or other fEOAD cells in total cell lysate but elevated in nuclear fraction (n = 3, * p < 0.05) (A). The proportion of GSK3β phosphorylated on serine 9, standardized to non-phosphorylated GSK3β, was substantially higher in A360T cells compared with healthy controls or other fEOAD cells (n = 3, * p < 0.05) (B). The amount of β-catenin and GSK3β forming a complex with PS1 was lower in A360T cells than healthy controls or other fEOAD cells, n = 3, * p < 0.05 (C). Conversely, the amount of the PS1-GSK3β(Ser9) complex was increased in A360T cells relative to that in healthy controls or other fEOAD cells (n = 3, * p < 0.05) (D).