TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant features | Reference or source |
|---|---|---|
| E. coli K-12 strains | ||
| DH5α | High-efficiency transformation | BRLa |
| SM10 | Tra functions of IncP plasmids integrated into chromosome | 35 |
| S17 | Tra functions of IncP plasmids integrated into chromosome | 35 |
| B. pertussis strains | ||
| Tohama I | Patient isolate | 23 |
| BP907 | Tohama I; Nalr Strr ΔbvgA | 40 |
| BP947 | Tohama I; Nalr StrrfhaB-lacZ | 37 |
| TM1081 | Tohama I; Nalr StrrfhaB-lacZ vrg6-phoA | 30 |
| TM1126 | TM1081-bvgR (in-frame insertional mutant) | 30 |
| TM1311 | ΔbvgA orf2-phoA | This study |
| TM1312 | ΔbvgA orf1-phoA (= bvgR-phoA) | This study |
| TM1315 | fhaB-lacZ orf2-phoA | This study |
| TM1316 | fhaB-lacZ orf1-phoA ( = bvgR-phoA) | This study |
| Plasmids | ||
| pBBRKAN | Replication in Bordetella; mobilizable by IncP Tra functions | 2 |
| pTM193 | pBBRKAN with multiple cloning site inserted | This study |
| pSS2000 | Apr GmrrpsL oriT cos | 30 |
a
BRL, Bethesda Research Laboratories.