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(a) Map of the 9.6-kb EcoRI fragment carring the prsDE genes. Downstream from prsDE and ORF3 and in the opposite orientation are four genes (pssFCDE) homologous to glycosyl transferases in R. leguminosarum bv. trifolii (45) and R. meliloti (19). Upstream from prsDE is plyA, encoding a putative polysaccharide lyase. (b) The plyB gene is located in a 3.6-kb EcoRI fragment. Tn5 insertions identified in prsD, plyA, and plyB are indicated by solid circles. E, EcoRI; B, BamHI.
