TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
| Strains | ||
| 8401 | R. leguminosarum lacking pSym; Strr | 24 |
| 8401(pRL1JI) | Derivative of 8401 carrying the symbiotic plasmid pRL1JI | 9 |
| A408 | 8401 prsD1::Tn5 | 16 |
| A412 | 8401(pRL1JI) prsD1::Tn5 | 16 |
| A501 | 8401(pRL1JI) plyA2::Tn5 | This work |
| A503 | 8401(pRL1JI) plyA1::Tn5 | This work |
| A575 | 8401(pRL1JI) prs6::Tn5 | This work |
| A590 | 8401(pRL1JI) prsD2::Tn5 | This work |
| A591 | 8401(pRL1JI) prsD3::Tn5 | This work |
| A599 | 8401(pRL1JI) prsD4::Tn5 | This work |
| A600 | 8401(pRL1JI) plyB1::Tn5 | This work |
| A616 | Transductant of 8401 with plyB1::Tn5 | This work |
| A617 | Transductant of 8401(pRL1JI) with plyB1::Tn5 | This work |
| A632 | 8401 plyA2::Tn5 | This work |
| A633 | 8401 plyA1::Tn5 | This work |
| A636 | 8401 plyA::SpcrplyB1::Tn5 | This work |
| A638 | 8401(pRL1JI) plyA3::Spcr | This work |
| A640 | 8401(pRL1JI) plyA3::SpcrplyB1::Tn5 | This work |
| Plasmids | ||
| pGV910-C1 | pGV910 derivative carrying truncated egl from A. caulinodans on a 4.3-kb EcoRI fragment from pRGC1 | 17 |
| pIJ7298 | pLAFR1 cosmid carrying prsDE and plyA | 16 |
| pIJ7349 | 9.6-kb EcoRI fragment from pIJ7298 carrying prsDE, in pIJ1891 | 16 |
| pIJ7419 | pIJ7298 derivative with plyA2::Tn5 | This work |
| pIJ7421 | pIJ7298 derivative with plyA1::Tn5 | This work |
| pIJ7474 | 16-kb EcoRI fragment from pIJ7419 carrying plyA2::Tn5 in pBluescript (SK+) | This work |
| pIJ7647 | pLAFR1 cosmid carrying plyB | This work |
| pIJ7653 | pIJ7647 derivative with plyB1::Tn5 | This work |
| pIJ7708 | 2-kb EcoRI-BamHI fragment with plyB from pIJ7647 in pUC18 | This work |
| pIJ7709 | 2-kb EcoRI-BamHI fragment with plyB from pIJ7647 in pIJ1891 | This work |
| pIJ7754 | 12-kb EcoRI fragment with plyA::Spcr in pBluescript(SK+) | This work |
| pIJ7765 | Derivative of pJQ200(KS) carrying 12-kb EcoRI fragment with plyA::Spcr from pIJ7754 | This work |
| pIJ7871 | plyA cloned behind a vector promoter in pKT230 | This work |
| pRGC1 | pLAFR1 cosmid carrying the egl endoglycanase gene from A. caulinodans | 17 |