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. 2023 Nov 21;15(23):4473. doi: 10.3390/polym15234473

Figure 5.

Figure 5

Cell viability test. (A) Assessment of C2C12 cell viability in structures printed with PCMC/Alg bioink using Live/Dead (green/red) assay. Live/Dead assays were conducted at predefined intervals: 1 day, 4 days, and 7 days after bioprinting. (B) Quantification of cell viability. The percentage was determined by dividing the number of live cells by the total cell count. ImageJ software was used to count live and dead cells from the images acquired through the Live/Dead assay. PCMC/Alg-2 and PCMC/Alg-3 with more porous structures may improve the exchange of growth factors and waste removal, resulting in higher cell viability. They can reduce shear stress when 3D bioprinting because of their lower elastic modulus. Error bars indicate the standard mean of errors. The same letter means statistical insignificance (Duncan’s new multiple range test, p ≤ 0.05).