FIG. 2.

Southern blot experiments for characterization of complete segregation of the gcp mutant. The ³²P-labelled internal BclI/KpnI fragment of the gcp gene (A) or the ³²P-labelled aphII gene (B) was used as a probe for the hybridization to EcoRI-BamHI- (lanes 1 and 2) and to HindIII-BamHI-digested (lanes 3 and 4) chromosomal DNA from the WT (lanes 1 and 3) and the gcp mutant (lanes 2 and 4), respectively. The molecular masses of HindIII-digested λ DNA size standards were drawn by using the positions from the photo of the ethidium bromide-stained gel at the same magnification.