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. 2023 Dec 8;9(49):eade1370. doi: 10.1126/sciadv.ade1370

Fig. 4. Monocolonization of neonatal SPF mice with BI or BT differentially affects developing immunity in spleen.

Fig. 4.

(A) Experimental approach: SPF mice were orally gavaged with either 108 CFUs of BI, heat-killed BI (HK_BI), or BT at day 7 of life. Control animals were colonized with PBS alone. Boost gavages of similar dosage were performed at day 10 of life. All animals were euthanized, and immunity was analyzed in spleen at day 14 of life. (B and C) Body weights and spleen counts. (D to F) Frequencies of Ly6Chigh and Ly6Clow monocytes and Ly6G+ neutrophils. (G to I) Proportions of effector memory, central memory, and naive CD4+ T cells. (J) Ct values with and without PMA treatment in BI-specific qPCR. (K) Ct values with and without PMA treatment in BT-specific qPCR. (L) Concentrations of lipocalin-2 in serum at day 14 of life. Data are representative of two independent experiments. Means ± SEM are plotted (*P < 0.05, **P < 0.01, and ***P < 0.001).