FIG. 3.
Infection of strains expressing TolA deletion mutant proteins. Cultures of K17DE3tolA/F+ bacteria containing plasmids expressing either TolAΔIIn (•), TolAΔIIc (□), TolAΔII (○), or no TolA (⧫) were infected for 15 min with 35S-labeled phages, and the membrane fractions of the washed cultures (approximately 3 × 105 cpm) were analyzed as described in the legend to Fig. 1. Radioactivity is expressed as a percentage of the total in the gradient. The inset is an expanded scale comparing radioactivity in fractions 10 to 21 for bacteria with TolAΔII (○) and for bacteria with no TolA (♦). The washed cultures contained 6.4, 6.0, 5.2, and 5.2% of the input radioactivity, respectively.