TABLE 1.

Fate of pVIII following infection of wild-type and tolA mutant E. coli

Expt	Straina	Label in phagesb	% Input label remaining with bacteria afterc:
			Washing	Shearing	Subtilisin
1	K17/F−	[3H]Lys	0.16
	K17/F+		3.98
	K17tolA/F+		2.20
2	K91	[3H]Lys	4.18	3.62
	K91tolA		3.35	0.85
3	K91	32P	8.35	6.16
	K91tolA		7.97	1.68
4	K17/F+	[35S]Met-[35S]Cys	3.84	2.65	2.57
	K17tolA/F+		2.61	0.47	0.35
5	K17/F+	[3H]Lys	2.24		2.24d
	K17tolA/F+		3.21		1.08d

^aAll K17 strains are K17DE3, as described in Materials and Methods. 

^bThe specific activity of the [³H]lysine-labeled phages was 2.5 to 3.5 × 10⁴ cpm/10¹⁰ PFU, that of the [³⁵S]methionine-[³⁵S]cysteine-labeled phages was 1.8 × 10⁴ cpm/10¹⁰ PFU, and that of the ³²P-labeled phages was 3.7 × 10³ cpm/10¹⁰ PFU. 

^cBacteria were grown to 2 × 10⁸ per ml, infected for 10 min with the appropriately labeled bacteriophage at a multiplicity of infection of 100, and harvested by centrifugation. The amount of radioactivity associated with the bacteria was measured after sequential washing, shearing, and treatment with subtilisin as described in Materials and Methods. 

^dWashed bacteria which were treated with subtilisin without shearing.