Figure 2.

The patient's serum was incubated with NA1/NA1- (top) or NA2/NA2 (bottom)-typed donor cells in buffer or quinidine solutions. NA1 and NA2 are granulocyte-specific antigens that differ in their amino acid sequence. Antibodies may be specific to one or the other antigen. Leukocyte-bound antibodies were detected with antihuman antibody labeled with fluoresceine. The ratios of the mean peak channel of the patient's serum are shown compared with that of the control serum for lymphocytes, monocytes, and granulocytes.