Alignment of promoter regions from rhizobial
rpoN genes. The aligned rpoN sequences are from
R. meliloti (Rm), R. etli (Re),
Rhizobium sp. strain NGR234, and B. japonicum
rpoN2 (BjrpoN2). The −10 and −35 regions from the R.
meliloli rpoN promoter are overlined, and the transcription start
site is indicated by an arrow (43). The inverse/complement
of the consensus (Cons) sequence of a −24/−12 promoter is indicated;
conserved GC and CC residues are in boldface. Nucleotides identical to
the consensus −24/−12 sequence are underlined. Ec, E. coli.