TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | Gibco/BRL | |
| HB101 | Smr | 45 |
| R. etli | ||
| CNPAF512 | Nalr, wild type | This work |
| FAJ1154 | NmrrpoN::Ω-Km, opposite orientation | This work |
| FAJ1155 | NmrrpoN::Ω-Km, same orientation | This work |
| FAJ1156 | NmrrpoN::gusA-aphII | This work |
| FAJ1157 | NmrrpoN::aphII-gusA | This work |
| FAJ1164 | NmrptsN::Ω-Km, same orientation | This work |
| FAJ1165 | NmrptsN::Ω-Km, opposite orientation | This work |
| FAJ1166 | NmrptsA::Tn5-mob | This work |
| R. meliloti | ||
| 2011 | Wild type | |
| 1681 | Smr KmrrpoN::Tn5 | 43 |
| Plasmids | ||
| pUC18 | Apr, cloning vector | 35 |
| pJQ200-UC1 | GmrsacB | 40 |
| pHP45Ω-Km | Apr Kmr | 14 |
| pWM6 | Apr NmruidA2 | 33 |
| pLAFR1 | Tcr, broad-host-range vector | 15 |
| pFAJ21 | Tcr, A. brasilense pnifH-gusA | 51 |
| pFAJ1150 | Tcr, rpoN gene in pLAFR1 | This work |
| pFAJ1152 | Apr, pUC18, rpoN 6.5-kb HindIII fragment | This work |
| pFAJ302 | Tcr, fusion between A. brasilense ammonium transporter amtB and gusA | Anne Van Dommelen (this laboratory) |